Typically, protein-HRP conjugates (such as streptavidin-HRP and antibodies-HRP) contain one to three monomeric HRP molecules per protein and do not contain polymerized HRP. This ratio of conjugation is effective in most routine biotin detection assays but may lack the sensitivity required for detecting targets present in low picogram to femtogram amounts without the use of additional time- and cost-consuming steps to amplify the signal
Relative signal intensity
Streptavidin Poly-HRP conjugate allows to obtain the highest sensitivity and low background level in immunoassays where the target molecule is presented at low levels or sample volume is limited. Scottsdalebio Streptavidin Poly-HRP is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.
As with all immunoassays, optimization is required to achieve the best signal-to-noise ratio. Switching to higher sensitivity substrates and conjugates can reveal more desired signal as well as background signal. If this occurs, follow standard primary and secondary dilution techniques or perform blocking buffer optimization.