Streptavidin poly-HRP is a HRP-conjugate specific for biotinylated antibodies and is manufactured by controlled polymerization of HRP molecules over the 5 nm globular core followed by covalent conjugation to streptavidin protein using proprietary conjugation technology.
|Amount: 0.5 mg||Storage Conditions: -20˚C|
|Working consentration:||Western blotting: 50 – 100 ng/ml|
|ELISA: 50 – 100 ng/ml||Immunohistochemistry: 5 – 100 µg/ml|
This product was purified by size exclusion chromatography and lyophilized in 10mM potassium phosphate buffer pH 6.5.
To prepare a working 0.5 mg/mL solution, resuspend the lyophilized Streptavidin poly-HRP in 1000 µL of 0.5× PBS pH 7.4 or in commercially available HRP stabilizer.
For long term storage, add 0.01% thiomersal or any other suitable preservative to the solution. Do not add sodium azide.
Store prepared solution at 4˚C. For long-term storage, aliquot and store at -20˚C.
Avoid repeated freeze-thaw cycles.