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Enhanced Streptavidin-HRP

Catalog# 4741

Enhanced Streptavidin-HRP

Streptavidin, poly-HRP linker

Streptavidin poly-HRP is a HRP-conjugate specific for biotinylated antibodies and is manufactured by controlled polymerization of HRP molecules over the 5 nm globular core followed by covalent conjugation to streptavidin protein using proprietary conjugation technology.

Amount: 0.6 mg Storage Conditions: -20˚C
Working consentration: Western blotting: 50 – 100 ng/ml
ELISA: 50 – 100 ng/ml Immunohistochemistry: 5 – 100 µg/ml
The optimal dilution must be determined empirically for each specific application.
Sample preparation

This product was purified by size exclusion chromatography and lyophilized in 10mM potassium phosphate buffer pH 6.5.
To prepare a working 0.5 mg/mL solution, resuspend the lyophilized Streptavidin poly-HRP in 1000 µL of 0.5× PBS pH 7.4 or in commercially available HRP stabilizer.
For long term storage, add 0.01% thiomersal or any other suitable preservative to the solution. Do not add sodium azide.
Store prepared solution at 4˚C. For long-term storage, aliquot and store at -20˚C.
Avoid repeated freeze-thaw cycles.

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